DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Stomach, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
New England Biolabs, Inc. Monarch® gDNA Cell Lysis Buffer - 20 ml
The Monarch gDNA Cell Lysis Buffer is a component of the Monarch Genomic DNA Purification Kit . It provides mild lysis conditions that help to reduce the viscosity common in cell samples. Optimized for speed and efficiency, this buffer requires only a 5 minute lysis incubation time with Monarch Proteinase K and Monarch RNase A to obtain maximal yields of highly pure and intact gDNA with a typical peak size >60 kb.
- A component of the Monarch Genomic DNA Purification Kit Provides mild lysis conditions that help to reduce the viscosity common in cell samples
- Requires only a 5 minute lysis incubation with Monarch Proteinase K and Monarch RNase A
- Monarch kit components are also available separately for added convenience
Zymo Research Corporation ZymoPURE P1 (Red), 410 ml
ZymoPURE P1 (Red) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
LGC GENOMICS LLC (Lucigen) sbeadex™ PCR Clean-up Kit, 96 purificationsHighly efficient, magnetic bead based purification of PCR products.
Highly efficient, magnetic bead based purification of PCR products.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Rice, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Genomic DNA Clean & Concentrator™ Kit-25 (25 Preps)
There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied ZymoSpin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product isalso compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions,crude plasmid preparations, cDNA synthesis, etc.
Biotium AccuGreen™ High Sensitivity dsDNA Quantitation Kit (100 assays)
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA. The assay has a green fluorescence with an excitation/emission of 502/523 nm (with DNA). The assay has a linear range of 0.1-100 ng dsDNA and a sample concentration range between 5 pg/uL and 100 ng/uL. This trial size kit has sufficient reagent for 100 assays.
New England Biolabs, Inc. NEBNext Ultra™ II FS DNA Module – 24 reactions
This module is part of the NEBNext Ultra II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module, for Illumina-compatible library construction.
Epoch Life Science Inc GenCatch™ Blood &Tissue Genomic Miniprep Kit (50 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast, cost-effective method to extract gDNA ready for downstream use from various starting materials (such as bacteria, yeast, blood, cells, tissue, feces, etc.). Silica membrane technology. 120 ug Binding capacity. Instructions for sample type listed in protocol. Used by labs around the world. 18 month shelf life.
Research Products International Corp Quick-DNA Miniprep Plus Kit, 200 Preps
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Quick & Easy: Simple 20 minute procedure.
Highest Yield: Recover 3x more DNA.
Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.