DNA Extraction and Purification
Zymo Research Corporation EZ-96 DNA Methylation-Direct MagPrep (4x96 rxns.)
The EZ-96 DNA Methylation-Direct MagPrep allows simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. These innovations provide high-throughput methylation analysis. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This kit has been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Thomas Scientific DirectAmpT Plant Genomic DNA Amplification Kit 10 reactions
DirectAmpT Plant Genomic DNA Amplification Kit 10 reactions
Takara Bio NucleoBond® Xtra Midi Plus, 50 preps
NucleoBond Xtra Midi Plus (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A.
Biotium Caldesmon, HMW (h-Caldesmon)(CALD1/820 + h-CALD), CF740 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Alkali Scientific Molecular Biology Kit, EZ 10 Spin Column Total RNA Miniprep Kit, 20 Preps
EZ 10 Spin Column Total RNA Miniprep Kit: The EZ‐10 Column Total RNA Mini‐Prep Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format. This kit simplifies total RNA isolation by combining the stringency of guanidine‐isothiocyanate lysis with the speed and purity of silica‐based purification. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide opmal binding condions. The lysate is then loaded onto the EZ‐10 column with a silica membrane. RNA binds to the silica membrane, all protein and other components are removed in the flow through. Remaining contaminants and salts are efficiently washed away. Purified RNA is eluted in RNase‐free water has OD260/OD280 raos of 1.9‐2.1 (measured in 10 mM TrisHCl, pH 7.5) and is ideal for use in most downstream applications including Northern blong, RT‐PCR, Quantave PCR, Poly (A) RNA selection and Array analys.
Biochain Institute Inc Control Genomic DNA - Guinea Pig Female, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Express Cloning Checker Kit II: Blue Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Sigma Aldrich Fine Chemicals Biosciences TempliPhi 500 Amplification Kit GE Healthcare, 25-6400-50
TempliPhi 500 Amplification Kit GE Healthcare, 25-6400-50